ProSci

CAS9 Antibody

Product Code:
 
PSI-15-823
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of wild HeLa cells and Hela transfected with cas9, using CAS9 antibody (15-823) at 1:5000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
2 / 2
Immunofluorescence analysis of HeLa cells transfected with Cas9 (upper left) and untreated HeLa cells (upper right) using CAS9 Rabbit pAb (red, 15-823) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of wild HeLa cells and Hela transfected with cas9, using CAS9 antibody (15-823) at 1:5000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of HeLa cells transfected with Cas9 (upper left) and untreated HeLa cells (upper right) using CAS9 Rabbit pAb (red, 15-823) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-15-823-50uL50uL£433.00
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Further Information

Additional Names:
SPy_1046, SPy1046, 0
Application Note:
WB: 1:5000 - 1:10000

IF: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of streptococcus pyogenes CAS9
NCBI Gene ID #:
901176
NCBI Official Name:
hypothetical protein
NCBI Official Symbol:
CAS9
NCBI Organism:
Staphylococcus aureus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 150kDa
Purification:
Affinity purification
Research Area:
Other
User NOte:
Optimal dilutions for each application to be determined by the researcher.