ProSci

ANXA5 Antibody

Product Code:
 
PSI-15-284
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using ANXA5 antibody (15-284) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunofluorescence analysis of HeLa cells using ANXA5 antibody (15-284). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using ANXA5 antibody (15-284) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of HeLa cells using ANXA5 antibody (15-284). Blue: DAPI for nuclear staining.

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PSI-15-284-50uL50uL£433.00
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Further Information

Additional Names:
ANXA5, PP4, ANX5, ENX2, RPRGL3, HEL-S-7
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human ANXA5 (NP_001145.1).
NCBI Gene ID #:
308
NCBI Official Name:
annexin A5
NCBI Official Symbol:
ANXA5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 36kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle
Swissprot #:
P08758
User NOte:
Optimal dilutions for each application to be determined by the researcher.