ProSci

EDC3 Antibody

Product Code:
 
PSI-15-190
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using EDC3 antibody (15-190) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 2
Immunohistochemistry of paraffin-embedded rat brain using EDC3 antibody (15-190) at dilution of 1:150 (40x lens).
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Western blot analysis of extracts of various cell lines, using EDC3 antibody (15-190) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using EDC3 antibody (15-190) at dilution of 1:150 (40x lens).

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PSI-15-190-50uL50uL£433.00
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Further Information

Additional Names:
Enhancer of mRNA-decapping protein 3, LSM16 homolog, YjeF N-terminal domain-containing protein 2, YjeF_N2, hYjeF_N2, YjeF domain-containing protein 1, EDC3, LSM16, YJDC, YJEFN2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 159-508 of human EDC3 (NP_079359.2).
NCBI Gene ID #:
80153
NCBI Official Name:
Enhancer of mRNA-decapping protein 3
NCBI Official Symbol:
EDC3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 56kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q96F86
User NOte:
Optimal dilutions for each application to be determined by the researcher.