ProSci

CAMLG Antibody

Product Code:
 
PSI-15-165
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using CAMLG antibody (15-165) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human breast cancer using CAMLG antibody (15-165) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using CAMLG antibody (15-165) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using CAMLG antibody (15-165) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using CAMLG antibody (15-165) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse testis using CAMLG antibody (15-165) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse brain using CAMLG antibody (15-165) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of NIH-3T3 cells using CAMLG Polyclonal Antibody (15-165) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2 OS cells using CAMLG Polyclonal Antibody (15-165) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using CAMLG antibody (15-165) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human breast cancer using CAMLG antibody (15-165) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using CAMLG antibody (15-165) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using CAMLG antibody (15-165) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using CAMLG antibody (15-165) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using CAMLG antibody (15-165) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using CAMLG antibody (15-165) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using CAMLG Polyclonal Antibody (15-165) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using CAMLG Polyclonal Antibody (15-165) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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PSI-15-165-50uL50uL£433.00
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Further Information

Additional Names:
CAMLG, CAML, MGC163197
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:200
Background:
The immunosuppressant drug cyclosporin A blocks a calcium-dependent signal from the T-cell receptor (TCR) that normally leads to T-cell activation. When bound to cyclophilin B, cyclosporin A binds and inactivates the key signaling intermediate calcineurin. The protein encoded by this gene functions similarly to cyclosporin A, binding to cyclophilin B and acting downstream of the TCR and upstream of calcineurin by causing an influx of calcium. This integral membrane protein appears to be a new participant in the calcium signal transduction pathway, implicating cyclophilin B in calcium signaling, even in the absence of cyclosporin.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human CAMLG (NP_001736.1).
NCBI Gene ID #:
819
NCBI Official Name:
calcium modulating ligand
NCBI Official Symbol:
CAMLG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 38kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
P49069
User NOte:
Optimal dilutions for each application to be determined by the researcher.