ProSci

ACAT1 Antibody

Product Code:
 
PSI-14-882
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines, using ACAT1 antibody (14-882) at 1:5000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 5
Immunohistochemistry of paraffin-embedded human liver damage using ACAT1 antibody (14-882) at dilution of 1:100 (40x lens).
3 / 5
Immunofluorescence analysis of C6 cells using ACAT1 antibody (14-882) at dilution of 1:100. Blue: DAPI for nuclear staining.
4 / 5
Immunofluorescence analysis of NIH-3T3 cells using ACAT1 antibody (14-882) at dilution of 1:100. Blue: DAPI for nuclear staining.
5 / 5
Immunofluorescence analysis of U-2 OS cells using ACAT1 antibody (14-882) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using ACAT1 antibody (14-882) at 1:5000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human liver damage using ACAT1 antibody (14-882) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using ACAT1 antibody (14-882) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using ACAT1 antibody (14-882) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using ACAT1 antibody (14-882) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-14-882-50uL50uL£433.00
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Further Information

Additional Names:
acetoacetyl Coenzyme A thiolase, THIL, T2, MAT, ACAT, acetyl-Coenzyme A acetyltransferase 1, ACAT1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-145 of human ACAT1 (NP_000010.1).
NCBI Gene ID #:
38
NCBI Official Name:
acetyl-CoA acetyltransferase 1
NCBI Official Symbol:
ACAT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 42kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P24752
User NOte:
Optimal dilutions for each application to be determined by the researcher.