ProSci

TRPA1 Antibody

Product Code:
 
PSI-14-496
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using TRPA1 antibody (14-496) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 2
Immunofluorescence analysis of mouse intestine using TRPA1 antibody (14-496) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using TRPA1 antibody (14-496) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of mouse intestine using TRPA1 antibody (14-496) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-14-496-50uL50uL£433.00
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This product comes from: United States.
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Further Information

Additional Names:
TRPA1, ANKTM1, TRPA-1
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
The structure of the protein encoded by this gene is highly related to both the protein ankyrin and transmembrane proteins. The specific function of this protein has not yet been determined; however, studies indicate the function may involve a role in signal transduction and growth control.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 850-950 of human TRPA1 (NP_015628.2).
NCBI Gene ID #:
8989
NCBI Official Name:
transient receptor potential cation channel, subfamily A, member 1
NCBI Official Symbol:
TRPA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 128kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
O75762
User NOte:
Optimal dilutions for each application to be determined by the researcher.