ProSci

IL17A Antibody

Product Code:
 
PSI-14-434
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using IL17A antibody (14-434) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
2 / 4
Immunohistochemistry of paraffin-embedded mouse kidney using IL17A antibody (14-434) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded rat kidney using IL17A antibody (14-434) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded human stomach using IL17A antibody (14-434) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using IL17A antibody (14-434) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse kidney using IL17A antibody (14-434) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using IL17A antibody (14-434) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using IL17A antibody (14-434) at dilution of 1:100 (40x lens).

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PSI-14-434-50uL50uL£433.00
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Further Information

Additional Names:
Interleukin-17A; CTLA-8; Cytotoxic T Lymphocyte-associated Antigen 8
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 24-155 of human IL17A (NP_002181.1).
NCBI Gene ID #:
3605
NCBI Official Name:
interleukin 17A
NCBI Official Symbol:
IL17A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 37kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Chemokines & Cytokines, Immunology, Innate Immunity
Swissprot #:
Q16552
User NOte:
Optimal dilutions for each application to be determined by the researcher.