ProSci

AKR7A2 Antibody

Product Code:
 
PSI-14-342
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using AKR7A2 antibody (14-342) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
2 / 3
Immunohistochemistry of paraffin-embedded mouse heart using AKR7A2 antibody (14-342) at dilution of 1:100 (40x lens).
3 / 3
Immunofluorescence analysis of HeLa cells using AKR7A2 antibody (14-342). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using AKR7A2 antibody (14-342) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
Immunohistochemistry of paraffin-embedded mouse heart using AKR7A2 antibody (14-342) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using AKR7A2 antibody (14-342). Blue: DAPI for nuclear staining.

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PSI-14-342-50uL50uL£433.00
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Further Information

Additional Names:
Aflatoxin B1 aldehyde reductase member 2, 111n11, AFB1 aldehyde reductase 1, AFB1-AR 1, Aldoketoreductase 7, Succinic semialdehyde reductase, SSA reductase, AKR7A2, AFAR, AFAR1, AKR7
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:10 - 1:100
Background:
The protein encoded by this gene belongs to the aldo/keto reductase (AKR) superfamily and AKR7 family, which are involved in the detoxification of aldehydes and ketones. The AKR7 family consists of 3 genes that are present in a cluster on the p arm of chromosome 1. This protein, thought to be localized in the golgi, catalyzes the NADPH-dependent reduction of succinic semialdehyde to the endogenous neuromodulator, gamma-hydroxybutyrate. It may also function as a detoxication enzyme in the reduction of aflatoxin B1 and 2-carboxybenzaldehyde. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 100-359 of human AKR7A2 (NP_003680.2).
NCBI Gene ID #:
8574
NCBI Official Name:
Aflatoxin B1 aldehyde reductase member 2
NCBI Official Symbol:
AKR7A2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 35kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
O43488
User NOte:
Optimal dilutions for each application to be determined by the researcher.