ProSci

TSPYL1 Antibody

Product Code:
 
PSI-14-295
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using TSPYL1 antibody (14-295) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
2 / 2
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug TSPYL1 antibody (14-295). Western blot was performed from the immunoprecipitate using TSPYL1 antibody (14-295) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using TSPYL1 antibody (14-295) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3s.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug TSPYL1 antibody (14-295). Western blot was performed from the immunoprecipitate using TSPYL1 antibody (14-295) at a dilition of 1:1000.

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PSI-14-295-50uL50uL£433.00
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Further Information

Additional Names:
Testis-specific Y-encoded-like protein 1, TSPY-like protein 1, TSPYL1, TSPYL
Application Note:
WB: 1:500 - 1:2000

IP: 1:50 - 1:100
Background:
The protein encoded by this gene is found in the nucleolus and is similar to that of a family of genes on the Y-chromosome. This gene is intronless. Defects in this gene are a cause of sudden infant death with dysgenesis of the testes syndrome (SIDDT).
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human TSPYL1 (NP_003300.1).
NCBI Gene ID #:
7259
NCBI Official Name:
Testis-specific Y-encoded-like protein 1
NCBI Official Symbol:
TSPYL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 60kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q9H0U9
User NOte:
Optimal dilutions for each application to be determined by the researcher.