ProSci

OPLAH Antibody

Product Code:
 
PSI-14-232
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using OPLAH antibody (14-232) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Western blot analysis of extracts of various cell lines, using OPLAH antibody (14-232) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.

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PSI-14-232-50uL50uL£433.00
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This product comes from: United States.
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Further Information

Additional Names:
5-Opase, OPLAD, OPLAH, 5-oxoprolinase, 5-oxo-L-prolinase, pyroglutamase
Application Note:
WB: 1:500 - 1:2000
Background:
The protein encoded by this gene acts as a homodimer, using ATP hydrolysis to catalyze the conversion of 5-oxo-L-proline to L-glutamate. Defects in this gene are a cause of 5-oxoprolinase deficiency (OPLAHD).
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1119-1288 of human OPLAH (NP_060040.1).
NCBI Gene ID #:
26873
NCBI Official Name:
5-oxoprolinase, ATP-hydrolysing
NCBI Official Symbol:
OPLAH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 134kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
O14841
User NOte:
Optimal dilutions for each application to be determined by the researcher.