ProSci

CAPN1 Antibody

Product Code:
 
PSI-14-117
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using CAPN1 antibody (14-117) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 2
Immunohistochemistry of paraffin-embedded mouse heart using CAPN1 antibody (14-117) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using CAPN1 antibody (14-117) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse heart using CAPN1 antibody (14-117) at dilution of 1:100 (40x lens).

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PSI-14-117-50uL50uL£433.00
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This product comes from: United States.
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Further Information

Additional Names:
CAPN1, calpain 1, (mu/I) large subunit, CANP, CANP1, CANPL1, muCANP, muCL, CANP 1, calcium-activated neutral proteinase 1, calpain mu-type, calpain, large polypeptide L1, calpain-1 catalytic subunit, calpain-1 large subunit, cell proliferation-inducing ge
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-330 of human CAPN1 (NP_005177.2).
NCBI Gene ID #:
823
NCBI Official Name:
calpain 1, (mu/I) large subunit
NCBI Official Symbol:
CAPN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 82kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Immunology, Neuroscience, Signal Transduction
Swissprot #:
P07384
User NOte:
Optimal dilutions for each application to be determined by the researcher.