ProSci

SRC Antibody

Product Code:
 
PSI-14-108
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines, using SRC antibody (14-108) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
2 / 5
Immunohistochemistry of paraffin-embedded human lung cancer using SRC antibody (14-108) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human breast cancer using SRC antibody (14-108) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded human placenta using SRC antibody (14-108) at dilution of 1:100 (40x lens).
5 / 5
Immunohistochemistry of paraffin-embedded human gastric cancer using SRC antibody (14-108) at dilution of 1:100 (40x lens).
❮❯

Western blot analysis of extracts of various cell lines, using SRC antibody (14-108) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human lung cancer using SRC antibody (14-108) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using SRC antibody (14-108) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human placenta using SRC antibody (14-108) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using SRC antibody (14-108) at dilution of 1:100 (40x lens).

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PSI-14-108-50uL50uL£433.00
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Further Information

Additional Names:
SRC, ASV, Pp60c-src, Tyrosine kinase pp60c-src, Tyrosine-protein kinase SRC-1, v-src, C-SRC, p60-Src, Proto-oncogene c-Src, SRC1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-80 of human SRC (NP_005408.1).
NCBI Gene ID #:
6714
NCBI Official Name:
v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog
NCBI Official Symbol:
SRC
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 60kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Immunology, Signal Transduction
Swissprot #:
P12931
User NOte:
Optimal dilutions for each application to be determined by the researcher.