ProSci

TREM2 Antibody

Product Code:
 
PSI-13-679
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of Mouse brain, using TREM2 antibody (13-679) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human tonsil using TREM2 antibody (13-679) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon carcinoma using TREM2 antibody (13-679) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using TREM2 antibody (13-679) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spleen using TREM2 antibody (13-679) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of U-251MG cells using TREM2 antibody (13-679) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of mouse lung cells using TREM2 antibody (13-679) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of Mouse brain, using TREM2 antibody (13-679) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human tonsil using TREM2 antibody (13-679) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using TREM2 antibody (13-679) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using TREM2 antibody (13-679) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using TREM2 antibody (13-679) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U-251MG cells using TREM2 antibody (13-679) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse lung cells using TREM2 antibody (13-679) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-13-679-50uL50uL£433.00
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Further Information

Additional Names:
TREM2, triggering receptor expressed on myeloid cells 2, TREM-2, Trem2a, Trem2b, Trem2c, OTTHUMP00000039771, triggering receptor expressed on myeloid cells 2a
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:200

IF: 1:20 - 1:100
Background:
This gene encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 19-160 of human TREM2 (NP_061838.1).
NCBI Gene ID #:
54209
NCBI Official Name:
triggering receptor expressed on myeloid cells 2
NCBI Official Symbol:
TREM2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 25kDa
Purification:
Affinity purification
Research Area:
Immunology, Innate Immunity
Swissprot #:
Q9NZC2
User NOte:
Optimal dilutions for each application to be determined by the researcher.