ProSci

PHGDH Antibody

Product Code:
 
PSI-13-666
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using PHGDH antibody (13-666) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
2 / 3
Immunohistochemistry of paraffin-embedded rat lung using PHGDH antibody (13-666) at dilution of 1:100 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded rat brain using PHGDH antibody (13-666) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using PHGDH antibody (13-666) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded rat lung using PHGDH antibody (13-666) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PHGDH antibody (13-666) at dilution of 1:100 (40x lens).

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PSI-13-666-50uL50uL£433.00
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Further Information

Additional Names:
PHGDH, 3-PGDH, 3PGDH, PGDH, Phosphoglycerate dehydrogenase, PDG, PGAD, PGDH3
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 264-533 of human PHGDH (NP_006614.2).
NCBI Gene ID #:
26227
NCBI Official Name:
phosphoglycerate dehydrogenase
NCBI Official Symbol:
PHGDH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 57kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
O43175
User NOte:
Optimal dilutions for each application to be determined by the researcher.