ProSci

SUCLA2 Antibody

Product Code:
 
PSI-13-392
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 1
Western blot analysis of extracts of various cell lines, using SUCLA2 antibody (13-392) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.

Western blot analysis of extracts of various cell lines, using SUCLA2 antibody (13-392) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.

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PSI-13-392-50uL50uL£433.00
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This product comes from: United States.
Typical lead time: 14-21 working days.
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Further Information

Additional Names:
A-BETA, A-SCS, MTDPS5, SCS-betaA, succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial, ATP-specific succinyl-CoA synthetase subunit beta, ATP-specific succinyl-CoA synthetase, beta subunit, mitochondrial succinyl-CoA ligase [ADP-forming] subunit beta, renal carcinoma antigen NY-REN-39, succinate-CoA ligase beta subunit, succinyl-CoA ligase [ADP-forming] subunit beta, mitochondrial, succinyl-CoA synthetase beta-A chain
Application Note:
WB: 1:500 - 1:1000
Background:
Succinyl-CoA synthetase (SCS) is a mitochondrial matrix enzyme that acts as a heterodimer, being composed of an invariant alpha subunit and a substrate-specific beta subunit. The protein encoded by this gene is an ATP-specific SCS beta subunit that dimerizes with the SCS alpha subunit to form SCS-A, an essential component of the tricarboxylic acid cycle. SCS-A hydrolyzes ATP to convert succinate to succinyl-CoA. Defects in this gene are a cause of myopathic mitochondrial DNA depletion syndrome. A pseudogene of this gene has been found on chromosome 6.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human SUCLA2 (NP_003841.1).
NCBI Gene ID #:
8803
NCBI Official Name:
succinate-CoA ligase ADP-forming beta subunit
NCBI Official Symbol:
SUCLA2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 50kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
Q9P2R7
User NOte:
Optimal dilutions for each application to be determined by the researcher.