ProSci

HDAC5 Antibody

Product Code:
 
PSI-13-228
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines, using HDAC5 antibody (13-228) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 5
Immunohistochemistry of paraffin-embedded rat liver using HDAC5 antibody (13-228) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human tonsil using HDAC5 antibody (13-228) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded mouse spinal cord using HDAC5 antibody (13-228) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of A-549 cells using HDAC5 antibody (13-228).
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Western blot analysis of extracts of various cell lines, using HDAC5 antibody (13-228) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat liver using HDAC5 antibody (13-228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using HDAC5 antibody (13-228) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spinal cord using HDAC5 antibody (13-228) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using HDAC5 antibody (13-228).

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PSI-13-228-50uL50uL£433.00
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Further Information

Additional Names:
HD5, NY-CO-9, NFKB3, HD5
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 550-650 of human HDAC5 (NP_005465.2).
NCBI Gene ID #:
10014
NCBI Official Name:
histone deacetylase 5
NCBI Official Symbol:
HDAC5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 132kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Immunology, Signal Transduction, Stem Cell
Swissprot #:
Q9UQL6
User NOte:
Optimal dilutions for each application to be determined by the researcher.