ProSci

PBEF / NAMPT Antibody

Product Code:
 
PSI-13-109
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines, using PBEF / NAMPT antibody (13-109) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 5
Immunohistochemistry of paraffin-embedded rat ovary using PBEF / NAMPT antibody (13-109) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human colon carcinoma using PBEF / NAMPT antibody (13-109) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded mouse spleen using PBEF / NAMPT antibody (13-109) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of HeLa cells using PBEF / NAMPT antibody (13-109).
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Western blot analysis of extracts of various cell lines, using PBEF / NAMPT antibody (13-109) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat ovary using PBEF / NAMPT antibody (13-109) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using PBEF / NAMPT antibody (13-109) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using PBEF / NAMPT antibody (13-109) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using PBEF / NAMPT antibody (13-109).

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PSI-13-109-50uL50uL£433.00
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Further Information

Additional Names:
PBEF1, 1110035O14Rik, DKFZP666B131, MGC117256, NAMPT, PBEF, VF, PBEF1, VISFATIN
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human PBEF / NAMPT (NP_005737.1).
NCBI Gene ID #:
10135
NCBI Official Name:
nicotinamide phosphoribosyltransferase
NCBI Official Symbol:
NAMPT
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 56kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Chemokines & Cytokines, Growth Factors, Immunology, Innate Immunity, Neuroscience, Signal Transduction
Swissprot #:
P43490
User NOte:
Optimal dilutions for each application to be determined by the researcher.