NSJ Bioreagents

Cleaved PARP1 Antibody

Product Code:
 
NSJ-RQ5288
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
HI-16
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store the Cleaved PARP antibody at -20°C.
1 / 2
Western blot testing of mouse 1) RAW264 and 2) NIH3T3 cell lysate with Cleaved PARP antibody. Predicted molecular weight ~24 kDa (DNA-binding domain).
2 / 2
IHC staining of FFPE human kidney tissue with Cleaved PARP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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Western blot testing of mouse 1) RAW264 and 2) NIH3T3 cell lysate with Cleaved PARP antibody. Predicted molecular weight ~24 kDa (DNA-binding domain).
IHC staining of FFPE human kidney tissue with Cleaved PARP antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

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NSJ-RQ5288-100ul100ul£535.00
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Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:100
Application Note:
Optimal dilution of the Cleaved PARP antibody should be determined by the researcher.
Description:
The main role of PARP (found in the cell nucleus) is to detect and initiate an immediate cellular response to metabolic, chemical, or radiation-induced single-strand DNA breaks (SSB) by signaling the enzymatic machinery involved in the SSB repair. [Wiki] Cleavage of PARP, by enzymes such as caspases or cathepsins, typically inactivates PARP. While in vitro cleavage by caspase occurs throughout the caspase family, preliminary data suggest that caspase-3 and caspase-7 are responsible for in vivo cleavage. Cleavage occurs at aspartic acid 214 and glycine 215, separating PARP into a 24kDA and 89kDA segment. The smaller moiety includes the zinc finger motif requisite in DNA binding. The 89 kDa fragment includes the auto-modification domain and catalytic domain. [Wiki]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human PARP (24kDa cleavage segment) was used as the immunogen for the Cleaved PARP antibody.
Limitation:
This Cleaved PARP antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
P09874