NSJ Bioreagents

HMGB1 Antibody

Product Code:
 
NSJ-RQ5274
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
AAC-8
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store the HMGB1 antibody at -20°C.
1 / 2
IHC staining of FFPE human kidney tissue with HMGB1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
2 / 2
Western blot testing of 1) human HeLa and 2) rat brain lysate with HMGB1 antibody. Predicted molecular weight ~25 kDa.
❮❯

IHC staining of FFPE human kidney tissue with HMGB1 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of 1) human HeLa and 2) rat brain lysate with HMGB1 antibody. Predicted molecular weight ~25 kDa.

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NSJ-RQ5274-100ul100ul£535.00
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Further Information

Application Details :
Western blot: 1:1000-1:2000,Immunohistochemistry (FFPE): 1:50-1:100
Application Note:
Optimal dilution of the HMGB1 antibody should be determined by the researcher.
Description:
High mobility group protein B1 is a multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. [UniProt]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human HMGB1 was used as the immunogen for the HMGB1 antibody.
Limitation:
This HMGB1 antibody is available for research use only.
Localization:
Nuclear, cytoplasmic, cell membrane, extracellular
Purity:
Affinity purified
Species Reactivity :
Human, Rat
Uniprot #:
P09429