NSJ Bioreagents

IL-34 Antibody

Product Code:
 
NSJ-RQ4931
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Antibody Clone:
 
n/a
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
After reconstitution, the IL-34 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Western blot testing of 1) human placenta, 2) rat brain and 3) mouse brain lysate with IL-34 antibody at 0.5ug/ml. Predicted molecular weight ~26 kDa, secreted as an ~39 kDa homodimer that may be glycosylated.
2 / 3
IHC staining of FFPE human tonsil tissue with IL-34 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
3 / 3
IHC staining of FFPE mouse spleen tissue with IL-34 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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Western blot testing of 1) human placenta, 2) rat brain and 3) mouse brain lysate with IL-34 antibody at 0.5ug/ml. Predicted molecular weight ~26 kDa, secreted as an ~39 kDa homodimer that may be glycosylated.
IHC staining of FFPE human tonsil tissue with IL-34 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE mouse spleen tissue with IL-34 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

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CodeSizePrice
NSJ-RQ4931-100ug100ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the IL-34 antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Interleukin 34 (IL-34) is a protein belonging to a group of cytokines called interleukins. It was originally identified in humans, by large scale screening of secreted proteins; chimpanzee, murine, rat and chicken interleukin 34 orthologs have also been found. The protein is composed of 241 amino acids, 39 kilodaltons in mass, and forms homodimers. IL-34 increases growth or survival of immune cells known as monocytes; it elicits its activity by binding the Colony stimulating factor 1 receptor. This gene is mapped to 16q22.1.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Amino acids NEPLEMWPLTQNEECTVTGFLRDKLQYR from the human protein were used as the immunogen for the IL-34 antibody.
Limitation:
This IL-34 antibody is available for research use only.
Localization:
Secreted
Purity:
Antigen affinity purified
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q6ZMJ4