NSJ Bioreagents

Phospho-EGF Receptor / EGFR Antibody (pY1092)

Product Code:
 
NSJ-RQ4842
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
BFO-5
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store the phospho-EGFR antibody at -20°C.
1 / 2
Western blot testing of lysate from HUVEC cells treated with EGF, with phospho-EGFR antibody. Expected molecular weight: 134-170 kDa depending on glycosylation level.
2 / 2
IHC staining of FFPE human breast cancer with phospho-EGFR antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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Western blot testing of lysate from HUVEC cells treated with EGF, with phospho-EGFR antibody. Expected molecular weight: 134-170 kDa depending on glycosylation level.
IHC staining of FFPE human breast cancer with phospho-EGFR antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

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NSJ-RQ4842-100ul100ul£535.00
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Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:200
Application Note:
Optimal dilution of the phospho-EGFR antibody should be determined by the researcher.
Description:
EGFR is a receptor tyrosine kinase. Receptor for epidermal growth factor (EGF) and related growth factors including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation.
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human EGFR (surrounding pY1092) was used as the immunogen for the phospho-EGFR antibody.
Limitation:
This phospho-EGFR antibody is available for research use only.
Localization:
Cell surface
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P00533