Mouse anti Human Myogenin
Antibody Clonality:
Monoclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Gel Shift
- Immunofluorescence (IF)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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This product comes from:
Netherlands.
Typical lead time:
7-10 working days.
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Further Information
Applications Description:
This antibody can be used for ELISA, gel supershift assays, immunofluorescence, immunoprecipitation (2 ?g/mg), Western blotting (1 ?g/ml) and immunohistochemistry on formalin-fixed, paraffin-embedded tissue sections (1-2 ?g/ml). Optimal concentration should be evaluated by serial dilutions.
Recognizes a protein of 34kDa, identified as myogenin. Its epitope localizes between aa 158-178 of myogenin. Myogenin is a member of a family of myogenic regulatory genes, which includes MyoD, myf5 and MRF4. These genes encode a set of transcription factors, which are essential for muscle development. Expression of myogenin is restricted to cells of skeletal muscle origin. It is therefore a useful marker for tumors of the muscle lineage, being strongly expressed in alveolar rhabdomyosarcomas. This antibody can immunoprecipitate protein-DNA complex and is the antibody of choice for staining of formalin-fixed, paraffin-embedded tissues.
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
See vial for concentration
Signal Transduction
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Western Blotting
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant protein containing rat myogenin amino acids 30-224 and mouse myeloma NS1 cells.
Rhabdomyosarcoma and Wlim's tumors
X1415M, clone F12B recognizes a protein of 34kDa, identified as myogenin. Its epitope localizes between aa 158-178 of myogenin.
Unconjugated
Products are stable for one year from purchase when stored properly
Protein A/G Chromatography
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant protein containing rat myogenin amino acids 30-224 and mouse myeloma NS1 cells.
Myogenin, Myogenic factor 4, Myf-4
P15173 (Human)P12979 (Mouse)P20428 (Rat)