Mouse anti Human CD45 conjugated with FITC

PBMC: Add 10 ?l of MAB/10^6 PBMC in 100 ?l PBS. Mix gently and incubate for 15 minutes at 2? to 8?C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 ?l of MAB/100 ?l of whole blood. Mix gently and incubate for 15 minutes at room temperature (20?C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer?s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjugates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm. RPE-Cy-5+: As above, however use only 5 ?l per test rather than 10 ?l. Excites at 488nm and emits at 670nm. Store protected from light.

Identification of all human leukocytes, lymphocytes, monocytes, polymorphonuclear cells, eosinophils and basophils in peripheral blood expressing the 180-220 kD M. W. T200 family surface antigen. CD45 is also present on thymus, spleen, tonsil and progenitor cells in bone marrow.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Titered for flow cytometry

Flow Cytometry

Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein

Flow Cytometry Staining

Derived from the hybridization of mouse NS-1 myeloma cells with spleen cells of BALB/c mice immunized with peripheral blood monocytes from rheumatoid arthritis patient

FITC

Reagents are stable for the period shown on the vial label when stored properly

Protein A/G Chromatography

CD45 FITC

P08575