Rabbit anti Human Caspase-9

Product Code:

EXA-A140P

Product Group:

Primary Antibodies

Supplier:

Nordic MuBio

Host Type:

Rabbit

Antibody Isotype:

IgG

Antibody Clonality:

Polyclonal

Regulatory Status:

RUO

Target Species:

Human

Shipping:

Ship at ambient temperature freeze upon arrival

Storage:

Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles

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Code	Size	Price

EXA-A140P

100 ug

£316.00

Quantity:

Prices exclude any Taxes / VAT

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Further Information

Applications Description:

Detects caspase-9 by Western blotting at 2-10 ?g/ml. Optimal concentration should be evaluated by serial dilutions.

Background:

Caspase-9 exists as a pro-form (mw ~46 kDa) that is cleaved by the activity of Apaf-1 in the presence of cytosolic cytochrome c (Apaf-2) and dATP. The processing of pro-caspase-9 into a heterodimer consisting of a 35kDa and a 10 kDa chain activates the protease and induces the cascade leading to cleavage of caspase-3 and other apoptotic targets. The processing of caspase-9 may be blocked by the activity of bcl-x which physically interacts with the caspase-/Apaf-1 complex.

Caution:

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.

Concentration:

See vial for concentration

Field of Interest:

Apoptosis

Formulation:

Provided as solution in phosphate buffered saline with 0.08% sodium azide

Functional Analysis:

Western Blotting

Immunogen:

Synthetic peptide corresponding to amino acids 301-313 of human caspase-9

Product Form:

Unconjugated

Product Stability:

Products are stable for one year from purchase when stored properly

Purification Method:

Ammonium Sulfate Precipitation

Synonyms:

Cysteine-requiring Aspartate Protease-9; iCE-like apoptotic protease 6;Apoptotic protease Mch-6; Apoptotic protease-activating factor 3

UniProt:

P55211

References

1. Duan H, et al., ICE-LAP6, a novel member of the ICE/Ced-3 gene family, is activated by the cytotoxic T cell protease granzyme B. J. Biol. Chem. 1996, 271, 16720-16724

2. Srinivasula SM, et al., The Ced-3/interleukin 1beta converting enzyme-like homolog Mch6 and the lamin-cleaving enzyme Mch2alpha are substrates for the apoptotic mediator CPP32. J. Biol. Chem. 1996, 271, 27099-27106

3. Li P, et al., Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 1997, 91, 479-489

4. Pan G, et al., Caspase-9, Bcl-XL, and Apaf-1 form a ternary complex. J. Biol. Chem. 1998, 273, 5841-5845