Nordic MuBio

Mouse anti HA Tag conjugated with agarose

Product Code:
 
EXA-X1029
Product Group:
 
Primary Antibodies
Supplier:
 
Nordic MuBio
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
12CA5
Regulatory Status:
 
RUO
Application:
 
Immunoprecipitation (IP)
Shipping:
 
Room Temperature
Storage:
 
Product should be stored at 4-8°C. DO NOT FREEZE
 

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EXA-X10291 ml£316.00
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Further Information

Applications Description:
Use 25?l of slurry/100?g of lysate for immunoprecipitation. Optimal concentration should be evaluated by serial dilutions.
Background:
A nine amino acid sequence derived from the viral hemagglutinin is frequently incorperated into recombinant proteins as an expression tag. Clone 12CA5 anti-HA is useful for western blotting and immunoprecipitation of these proteins. Shown is a typical western blot (10% SDS-PAGE) of HA-tagged Rit, a Ras-family GTPase, expressed in HEK293 cells and immunoprecipitated with anti-HA agarose (lane 1), lysate of HA-tagged Rit expressed in HEK293 cells (lane 2), and control HEK293 cells (lane 3) probed with clone 14G7 anti-Rit. Reacts with HA tagged proteins.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
Concentration:
See vial for concentration
Field of Interest:
Molecular Biology
Formulation:
Provided as 2x slurry (0.5 ml of settled gel) containing 20 mM sodium phosphate, 150 mM sodium chloride, pH 7.5, and 3 mM sodium azide
Functional Analysis:
Western Blotting
Immunogen:
Balb/C mice were immunized with a 9 amino acid petide to influenza virus hemagglutinin
Positive Control:
HA taged proteins
Product:
Provided as 2x slurry (0.5 ml of settled gel) containing 20 mM sodium phosphate, 150 mM sodium chloride, pH 7.5, 0.08% sodium azide
Product Form:
Agarose
Product Stability:
Reagents are stable for the period shown on the vial label when stored properly
Source:
Balb/C mice were immunized with a 9 amino acid petide of influenza virus hemagglutinin
Synonyms:
Mouse anti HA, Agarose Conjugate, hemaglutinin
UniProt:
Q3TBD2