ProSci

FITC Antibody [SPM395]

Product Code:
 
PSI-33-709
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SPM395
Regulatory Status:
 
RUO
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
 

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PSI-33-709-100ug100ug£765.00
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Further Information

Application Note:
Confocal Microscopy: suitable

Flow Cytometry: 0.5-1ug/10^6 cells in 0.1ml

IF: 0.5-1 ug/ml

WB: 0.5-1 ug/ml

IHC (FFPE): 0.5-1 ug/ml for 30 min at RT

Optimal dilution of the FITC antibody should be determined by the researcher.
Background:
This mAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxy-fluorescein. Sensitivity of the FITC-anti-FITC system is reportedly similar to that of the biotin-avidin system. In biotin or enzyme-labeled form, this mAb is also suitable for the conversion of fluorescein-labeled detection into immuno-enzymatic assays.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
FITC conjugated to KLH was used as the immunogen for this FITC antibody.
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Other
User NOte:
Optimal dilutions for each application to be determined by the researcher