Further Information
Mast/stem cell growth factor receptor Kit, SCFR, Piebald trait protein, PBT, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, CD117, KIT, SCFR
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
IF: 0.5-1 ug/ml
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD117 antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
This antibody recognizes a protein of 145kDa, identified as CD117, or p145kit. It is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, CD117 antibody has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi?s sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. CD117 antibody is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
0.2 mg/mL
Unconjugated
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Recombinant human protein was used as the immunogen for this CD117 antibody.
3815
KIT proto-oncogene receptor tyrosine kinase
CD117
Homo sapiens
Liquid
Protein G affinity chromatography
Cancer,Cell Cycle,Immunology,Neuroscience,Signal Transduction
P10721
Optimal dilutions for each application to be determined by the researcher