ProSci

IgG4 Antibody [IGHG4/1345]

Product Code:
 
PSI-33-799
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
IGHG4/1345
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
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IHC testing of human tonsil with IgG4 antibody (clone IGHG4/1345). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.
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IHC testing of human tonsil with IgG4 antibody (clone IGHG4/1345). HIER: boil sections in 10mM Tris with 1mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.

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PSI-33-799-100ug100ug£765.00
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Further Information

Application Note:
Flow Cytometry: 0.5-1ug/10^6 cells

IF: 1-2 ug/ml

WB: 0.5-1 ug/ml

IHC (FFPE): 0.5-1 ug/ml for 30 min at RT

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the IgG4 antibody to be titered up or down for optimal performance.
Background:
The regions of relatively constant sequence beyond the variable regions of immunoglobulins are termed constant regions (C regions) and are present in both the heavy and light chains. With very few exceptions, the sites of attachment for carbohydrates on immunoglobulins are located in these C regions. These regions also function to hold the variable regions together by using the disulfide bond between them. The C regions facilitate interaction with the antigen by increasing the maximum rotation of the immunoglobulin arms. Reportedly, a large population of patients with recurrent respiratory tract infection has low IgG4 concentrations. IgG4-related sclerosing disease has been recognized as a systemic disease entity characterized by an elevated serum IgG4 level, sclerosing fibrosis, and diffuse lymphoplasmacytic infiltration with the presence of many IgG4-positive plasma cells. IgG4 is overexpressed in inflammatory pseudotumor (IPT) and under expressed in inflammatory myofibroblastic tumor (IMT). In pulmonary nodular lymphoid hyperplasia (PNLH), there are an increased number of IgG4+ plasma cells.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A human recombinant protein corresponding to the Fc region was used as the immunogen for this IgG4 antibody.
NCBI Gene ID #:
3503
NCBI Official Name:
immunoglobulin heavy constant gamma 4 (G4m marker)
NCBI Official Symbol:
IGHG4
NCBI Organism:
homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Immunology
Swissprot #:
P01861
User NOte:
Optimal dilutions for each application to be determined by the researcher