ProSci

Thrombomodulin Antibody [THBD/1782]

Product Code:
 
PSI-33-688
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2a, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
THBD/1782
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
1 / 3
IHC testing of FFPE human cervical carcinoma with Thrombomodulin antibody (clone THBD/1782). HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.
2 / 3
3 / 3
❮❯

IHC testing of FFPE human cervical carcinoma with Thrombomodulin antibody (clone THBD/1782). HIER: boil tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9, for 10-20 min followed by cooling at RT for 20 min.

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-33-688-100ug100ug£765.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Note:
Flow Cytometry: 0.5-1ug/10^6 cells in 0.1ml

IF: 0.5-1 ug/ml

IHC (FFPE): 1-2 ug/ml for 30 min at RT

ELISA: 2-4 ug/ml; order the BSA-free formulation for coating

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Thrombomodulin antibody to be titered up or down for optimal performance.
Background:
It recognizes a protein of 75kDa, identified as Thrombomodulin. Thrombomodulin is a transmembrane glycoprotein with natural anticoagulant properties. It is normally expressed by a restricted number of cells, such as endothelial and mesothelial cells. In addition, synovial lining and syncytio-trophoblasts of placenta also express thrombomodulin. This protein is present in almost all of benign vascular tumors and majority of malignant vascular tumors (Kaposi s sarcoma, angiosarcoma, and epithelioid hemangioendothelioma). Hence, anti-thrombomodulin serves as a sensitive marker for lymphatic endothelial cells and their tumors. Recently, thrombomodulin antibody has been used for mesothelial cells and malignant mesotheliomas.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Human recombinant partial protein corresponding to amino acids 69-194 was used as the immunogen for this Thrombomodulin antibody.
NCBI Gene ID #:
7056
NCBI Official Name:
thrombomodulin
NCBI Official Symbol:
THBD
NCBI Organism:
homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Stem Cell
Swissprot #:
P07204
User NOte:
Optimal dilutions for each application to be determined by the researcher