ProSci

CD8a Antibody [3-298] (Cy5)

Product Code:
 
PSI-99-324
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
[3-298]
Regulatory Status:
 
RUO
Target Species:
  • Chicken (Gallus)
  • Turkey
Application:
 
Flow Cytometry
Shipping:
 
blue ice
Storage:
 
Store vial at 2-8˚C
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Chicken peripheral blood mononuclear cells were stained with Mouse Anti-Chicken CD8-CY5 (Cat. No. 99-324) and Mouse Anti-Chicken CD4-FITC .
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Chicken peripheral blood mononuclear cells were stained with Mouse Anti-Chicken CD8-CY5 (Cat. No. 99-324) and Mouse Anti-Chicken CD4-FITC .

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PSI-99-324-0.1mg0.1mg£686.00
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Further Information

Application Note:
CD8a Antibody [3-298] for use in flow cytometry, immunohistochemistry, and western blotting assays.
Background:
In the chicken the CD8 molecule is present in two forms - (i) a homodimer of two alpha chains and (ii) a heterodimer of an alpha chain and a beta chain. Chicken CD8 is expressed on approximately 80% of thymocytes, 45% of blood mononuclear cells, and 50% of spleen cells but less than 1% of cells in the bursa and bone marrow. While the vast majority of CD8+ cells in the thymus, spleen, and blood of adult chickens express both CD8alpha- and CD8beta-chains, a relatively large proportion of the CD8+ TCRgammadelta cells in the spleens of embryos and young chicks express only the alpha-chain of CD8. Among intestinal epithelial lymphocytes, the major CD8+ T cell populations present in mice are conserved but there is a population of TCRgammadelta CD8alphabeta cells in the chicken that is not found in rodents. The monoclonal antibody 3-298 recognizes the CD8alpha chain of both the chicken and turkey.
Buffer:
Supplied in PBS/NaN3
Concentration:
0.1 mg/mL
Conjugate:
Cy5
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Chicken thymocytes
NCBI Gene ID #:
403158
NCBI Official Name:
CD8a molecule
NCBI Official Symbol:
CD8A
NCBI Organism:
Gallus gallus
Physical State:
Liquid
Research Area:
Cancer,Immunology,Signal Transduction
SPECIFICITY:
CD8alpha
User NOte:
Optimal dilutions for each application to be determined by the researcher.