ProSci

TMEM16A Antibody [DG1/1486]

Product Code:
 
PSI-33-817
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2b, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
DG1/1486
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
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IHC testing of FFPE human gastrointestinal stromal tumor (GIST) with DOG1 antibody (DG1/1486). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.
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IHC testing of FFPE human gastrointestinal stromal tumor (GIST) with DOG1 antibody (DG1/1486). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.

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PSI-33-817-100ug100ug£765.00
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Further Information

Additional Names:
TMEM16A Antibody: DOG1, TAOS2, ORAOV2, TMEM16A, DOG1, Anoctamin-1, Discovered on gastrointestinal stromal tumors protein 1
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

IF: 0.5-1 ug/ml

IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the DOG1 antibody to be titered up or down for optimal performance.
Background:
Expression of DOG-1 protein is elevated in the gastrointestinal stromal tumors (GIST s), c-kit signaling-driven mesenchymal tumors of the GI tract. DOG-1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG-1 has been reported in 97.8 percent of scorable GIST s, including all c-kit negative GIST s. Overexpression of DOG-1 has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of DOG1 and c-kit in GIST s is nearly identical: 94.4% vs. 94.7%.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A recombinant human protein corresponding to amino acids 2-101 of human DOG1 was used as the immunogen for this DOG1 antibody.
NCBI Gene ID #:
55107
NCBI Official Name:
anoctamin 1, calcium activated chloride channel
NCBI Official Symbol:
ANO1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Signal Transduction
Swissprot #:
Q5XXA6
User NOte:
Optimal dilutions for each application to be determined by the researcher