ProSci

HER-2 Antibody [ERB2/776]

Product Code:
 
PSI-33-272
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
ERB2/776
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at 2-8C. Avoid freez-thaw cycles.
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FFPE staining of human breast carcinoma with HER-2 antibody (ERB2/776).
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FFPE staining of human breast carcinoma with HER-2 antibody (ERB2/776).

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PSI-33-272-100ug100ug£765.00
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Further Information

Additional Names:
erbB-2 Antibody: NEU, NGL, HER2, TKR1, CD340, HER-2, MLN 19, HER-2/neu, MLN19, NEU, Receptor tyrosine-protein kinase erbB-2, Metastatic lymph node gene 19 protein
Application Note:
Flow Cytometry: 0.5-1 ug/million cells

IF: 0.5-1 ug/ml

IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the HER-2 antibody to be titered up or down for optimal performance.
1. FFPE staining requires boiling tissue sections in 10mM Citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
2. This HER2 antibody is specific to the intracellular region of the protein.
Background:
ErbB2, also called HER2, is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. This ErbB2 antibody shows minimal cross-reaction with other members of the family. The protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant human protein (intracellular domain) was used as the immunogen for this HER-2 antibody. (2)
NCBI Gene ID #:
2064
NCBI Official Name:
erb-b2 receptor tyrosine kinase 2
NCBI Official Symbol:
Her2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer
Swissprot #:
P04626
User NOte:
Optimal dilutions for each application to be determined by the researcher