ProSci

MUC1 Antibody [SPM132]

Product Code:
 
PSI-34-133
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SPM132
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
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Formalin-fixed, paraffin-embedded human breast cancer stained with EMA antibody (SPM132).
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Formalin-fixed, paraffin-embedded human breast cancer stained with EMA antibody (SPM132).

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PSI-34-133-100ug100ug£765.00
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Further Information

Additional Names:
MUC1 Antibody : EMA, MCD, PEM, PUM, KL-6, MAM6, MCKD, PEMT, CD227, H23AG, MCKD1, MUC-1, ADMCKD, ADMCKD1, CA 15-3, MUC-1/X, MUC1/ZD, MUC-1/SEC, Mucin-1, Breast carcinoma-associated antigen DF3
Application Note:
Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

Immunofluorescence: 0.5-1 ug/ml

Immunohistochemistry (FFPE): 0.1-0.2 ug/ml for 30 min at RT (1)

Prediluted format: incubate for 30 min at RT (2)

The optimal dilution of the EMA antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
Epithelial Marker Antigen is a 265-400kDa glycoprotein. The alpha subunit has cell adhesive properties. It can act both as an adhesion and an anti-adhesion protein. EMA may provide a protective layer on epithelial cells against bacterial and enzyme attack. The beta subunit contains a C-terminal domain, which is involved in cell signaling, through phosphorylations and protein-protein interactions. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Human milk fat globule membranes were used as the immunogen for this EMA antibody. This mAb reacts with the DTRP epitope in the tandem repeats.
NCBI Gene ID #:
4582
NCBI Official Name:
mucin 1, cell surface associated
NCBI Official Symbol:
MUC1
NCBI Organism:
homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Signal Transduction
Swissprot #:
P15941
User NOte:
Optimal dilutions for each application to be determined by the researcher