ProSci

CD8 Antibody [C8/1035]

Product Code:
 
PSI-33-279
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
C8/1035
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
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SDS-PAGE Analysis of Purified, BSA-Free Anti-CD8 Antibody (clone C8/1035). Confirmation of Integrity and Purity of the Antibody.
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SDS-PAGE Analysis of Purified, BSA-Free Anti-CD8 Antibody (clone C8/1035). Confirmation of Integrity and Purity of the Antibody.

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PSI-33-279-100ug100ug£765.00
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Further Information

Additional Names:
T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2, CD8a, CD8A, MAL
Application Note:
Flow Cytometry: 0.5-1 ug/million cells

Immunofluorescence: 0.5-1 ug/ml



The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-CD8 antibody to be titered up or down for optimal performance.
Background:
Cluster of Differentiation 8 is a cell surface receptor expressed either as a heterodimer with the beta chain or as a homodimer. A majority of thymocytes and a subpopulation of mature Tcells and NK cells express CD8a. It binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in Tcell development and activation of mature Tcells. For mature Tcells, CD4 and 8 are mutually exclusive, so antibody to CD8 is generally used in conjunction with antibody to CD4.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant full-length human CD8a protein was used as immunogen for this anti-CD8 antibody.
NCBI Gene ID #:
925
NCBI Official Name:
CD8a molecule
NCBI Official Symbol:
CD8A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer,Immunology,Signal Transduction
Swissprot #:
P01732
User NOte:
Optimal dilutions for each application to be determined by the researcher