ProSci

MCM7 Antibody [MCM7/1466]

Product Code:
 
PSI-33-725
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2b
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
MCM7/1466
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
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Western blot testing of human 1) HeLa, 2) Raji and 3) HepG2 cell lysate with MCM7 antibody (clone MCM7/1466). Expected molecular weight: 80-90 kDa.
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Western blot testing of human 1) HeLa, 2) Raji and 3) HepG2 cell lysate with MCM7 antibody (clone MCM7/1466). Expected molecular weight: 80-90 kDa.

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PSI-33-725-100ug100ug£765.00
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Further Information

Additional Names:
DNA replication licensing factor MCM7, CDC47 homolog, P11-MCM3, MCM7, CDC47, MCM2
Application Note:
IF: 0.5-1 ug/ml

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

WB: 0.5-1 ug/ml

IHC (FFPE): 0.5-1 ug/ml for 30 min at RT

The stated application concentrations are suggested starting amounts. Titration of the MCM7 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Background:
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. [UniProt]
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Amino acids 195-319 were used as the immunogen for the MCM7 antibody.
NCBI Gene ID #:
4176
NCBI Official Name:
minichromosome maintenance complex component 7
NCBI Official Symbol:
MCM7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Other
Swissprot #:
P33993
User NOte:
Optimal dilutions for each application to be determined by the researcher