ProSci

EGFR Antibody [GFR450]

Product Code:
 
PSI-33-119
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG2a, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
GFR450
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
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IHC testing of squamous cell carcinoma stained with EGFR antibody (GFR450).
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SDS-PAGE Analysis of Purified, BSA-Free EGFR Antibody (clone GFR450). Confirmation of Integrity and Purity of the Antibody.
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IHC testing of squamous cell carcinoma stained with EGFR antibody (GFR450).
SDS-PAGE Analysis of Purified, BSA-Free EGFR Antibody (clone GFR450). Confirmation of Integrity and Purity of the Antibody.

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PSI-33-119-100ug100ug£765.00
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Further Information

Additional Names:
Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1
Application Note:
IHC (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)

Flow Cytometry: 0.5-1 ug/million cells in 0.1ml

IF: 1-2 ug/ml

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EGFR antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Background:

This antibody recognizes a protein of 170kDa, identified as EGFR.?EGFR?is type I receptor tyrosine kinase with sequence homology to?ErbB2, -3 -4 or?HER2, -3 -4. It binds to Epidermal Growth Factor (EGF),?Transforming Growth Factor-a?(TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head/neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
The immunizing protein for this antibody was recombinant human EGFR.
NCBI Gene ID #:
1956
NCBI Official Name:
epidermal growth factor receptor
NCBI Official Symbol:
EGF R
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Cancer,Signal Transduction
Swissprot #:
P00533
User NOte:
Optimal dilutions for each application to be determined by the researcher