NSJ Bioreagents

ZO-1 Antibody

Product Code:
 
NSJ-R31647
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the ZO-1 antibody can be stored for up to one month at 40. For long-term, aliquot and store at -200. Avoid repeated freezing and thawing.
1 / 5
Western blot testing of ZO-1 antibody and Lane 1: rat liver; 2: mouse liver; 3: (r) NRK; 4: (r) PC12; 5: human HeLa; 6: (h) SMMC-7721; 7: (m) HEPA; 8: (h) COLO320 lysate. Predicted molecular weight: ~194 kDa.
2 / 5
Western blot testing of human 1) A431, 2) Caco-2, 3) U-2 OS, 4) K562, 5) PC3, 6) T-47D, 7) rat ovary and 8) mouse ovary lysate with ZO-1 antibody. Predicted/observed molecular weight: ~194 kDa.
3 / 5
IHC-P: ZO-1 antibody testing of human intestinal cancer tissue. HIER: steamed with pH6 citrate buffer.
4 / 5
Immunofluorescent staining of FFPE human A431 cells with ZO-1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
5 / 5
Flow cytometry testing of human K562 cells with ZO-1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ZO-1 antibody.
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Western blot testing of ZO-1 antibody and Lane 1: rat liver; 2: mouse liver; 3: (r) NRK; 4: (r) PC12; 5: human HeLa; 6: (h) SMMC-7721; 7: (m) HEPA; 8: (h) COLO320 lysate. Predicted molecular weight: ~194 kDa.
Western blot testing of human 1) A431, 2) Caco-2, 3) U-2 OS, 4) K562, 5) PC3, 6) T-47D, 7) rat ovary and 8) mouse ovary lysate with ZO-1 antibody. Predicted/observed molecular weight: ~194 kDa.
IHC-P: ZO-1 antibody testing of human intestinal cancer tissue. HIER: steamed with pH6 citrate buffer.
Immunofluorescent staining of FFPE human A431 cells with ZO-1 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Flow cytometry testing of human K562 cells with ZO-1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ZO-1 antibody.

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CodeSizePrice
NSJ-R31647-100ug100 ug£535.00
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Further Information

Application Details :
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml,Immunofluorescence: 2-4ug/ml,Flow cytometry: 1-3ug/million cells
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the ZO-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
Zonula occludens protein 1, also called Tight junction protein 1, is a protein that in humans is encoded by the TJP1 gene. It is mapped to 15q13.1. This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions (zonulae occludentes). ZO-1 may be involved in signal transduction at cell?cell junctions. It has been found that injected CagA associates with the epithelial tight-junction scaffolding protein TJP1/ZO-1 and the transmembrane protein junctional adhesion molecule, causing an ectopic assembly of tight junction components at sites of bacterial attachment, and altering the composition and function of the apical-junctional complex.
Format :
Antigen affinity purified
Formulation :
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Gene ID #:
7082
Immunogen:
Human partial recombinant protein (AA 1178-1527) was used as the immunogen for this ZO-1 antibody.
Limitation:
This ZO-1 antibody is available for research use only.
Purity:
Antigen affinity
Species Reactivity :
Human, Mouse, Rat