AdipoGen Life Sciences

B-Xpander™ CD40L (human) (rec.) (Animal Free)

Product Code:
 
AG-40B-0010AF
Product Group:
 
Other Proteins
Host Type:
 
CHO Cells
Regulatory Status:
 
RUO
Shipping:
 
BI
Storage:
 
Short term: +4°C. Long term: -20°C
 

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CodeSizePrice
AG-40B-0010AF-C01010 ug£320.00
Quantity:
AG-40B-0010AF-C100100 ug£1,510.00
Quantity:
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This product comes from: Switzerland.
Typical lead time: 7-10 working days.
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Further Information

Alternate Names/Synonyms:
MultimericCD40L™ (Animal Free); ACRP30headless:CD40L; ACRP30headless:CD154; ADIPOQ-CD40L; ACRP30headless:TNFSF5
Biological Activity:
Induces B cell activation (as demonstrated by dose-dependent upregulation of CD86) (ED50: <1ng/ml).
Concentration:
After reconstitution: for 10ug size: 0.1mg/ml, for 100ug size: 1mg/ml
Endotoxin:
<0.01EU/ug purified protein (LAL test, Lonza).
Formulation:
Lyophilized. Contains PBS
Handling Advice:
Avoid freeze/thaw cycles.
Long Description:
CD40 ligand (CD40L) expressed by activated T cells engages CD40 expressed by B cells and APCs. CD40L can provide signals to B cells that induce proliferation, immunoglobulin class switching and antibody secretion. CD40L has been used recently in Immunotherapy to activate tumor-infiltrated lymphocytes (TIL). MultimericCD40L™ is a high-activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo. It provides a simple and equally potent alternative to [CD40L+enhancer] combinations. MultimericCD40L™ has been shown to be a potent tool for B cell expansion. It also has big potential as a growth factor for tumor-infiltrating lymphocytes (TILs), which have been shown to be important for T cell therapy. The CD40L (human) (multimeric) (rec.) (Animal Free) has been produced in CHO in suspension using animal-component free medium and certified CHO cells.
Molecular Weight:
~35-40kDa (SDS-PAGE)
Purity:
>95% (SDS-PAGE)
Sequence:
Human CD40L (aa 116-261) is fused at the N-terminus to mouse ACRP30headless?(aa 18-111) and a FLAG®-tag.
Source / Host:
CHO cells. Produced using animal component-free medium.
Species Crossreactivity:
Human
Specificity:
Fc (LALA-PG)-KIH (human) does not bind to FcRs.
Use & Stability:
Working aliquots are stable for up to 3 months when stored at -20°C.

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